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Merck & Co poly l ornithine hydrobromide
Poly L Ornithine Hydrobromide, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+l+ornithine/l+ornithine+poly/pm41538327-229-16-18
Average 86 stars, based on 1 article reviews
poly l ornithine hydrobromide - by Bioz Stars, 2026-10
86/100 stars

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Article Title: IGF2BPs directly regulate the noncanonical translation of toxic proteins from mutant FMR1 mRNA containing expanded CGG repeats.
Article Snippet: Briefly, 2 million iPSCs were plated on one Geltrex-coated well (Thermo Fisher Scientific) of 6-well plate in Essential 8 medium (Gibco) with 10 μM Y-27632 dihydrochloride (MedChemExpress).

Article Title: IGF2BPs directly regulate the noncanonical translation of toxic proteins from mutant FMR1 mRNA containing expanded CGG repeats.
Article Snippet: iPSCs were cultured in StemFlex (Thermo Fisher Scientific) medium on Geltrex-coated (Thermo Fisher Scientific) plates at 37 °C humidified incubator containing 5% CO2.

Imaging:

Article Title: A ligand/receptor trafficking clock governs self-renewal and abscission dynamics in pluripotent stem cells
Article Snippet: .. A total of 200,000 Neo1–Turbo-GFP or Unc5b–mKate cells were seeded onto 35 mm ibidi dishes precoated with poly-L-ornithine (Merck) 48 h prior to imaging. .. Time-lapse experiments were performed using the Nanolive 3D Explorer system, with fluorescence images excitation at 488 nm (Turbo-GFP) or 555 nm (mKate) together with bright-field images, every 10 min over a 24 h period.

Cell Culture:

Article Title: A ligand/receptor trafficking clock governs self-renewal and abscission dynamics in pluripotent stem cells
Article Snippet: .. α-tagged Netrin-1 cells were plated at 0.1M cells 48h before the start of the film in 35mm ibidi dishes coated with poly-L-ornithine (Merck) and cultured in medium containing 1ug/ml doxycycline. .. 1h before the start of the film, we removed the medium and incubated the cells for 1h on ice with the antibody solution containing FluoTag-X2 anti-ALPHA single domain antibody coupled with AF647 diluted 1:500 (Abnova ref RAB00922) and lysotracker ex/em 504/511 diluted 1:1000 (life technology ref L7526) in cold PBS1X.

Article Title: Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain
Article Snippet: LUHMES cells were obtained from ATCC (CRL-2927; RRID: CVCL_B056 ) and tested negative for mycoplasma contamination. .. Cells were cultured on 50 μg/mL Poly-L-ornithine (Merck) and 1 μg/mL human plasma fibronectin (Sigma) coated flasks and cultured in Advanced DMEM/F12 (Gibco) added with 1% N-2 supplement (Gibco), GlutaMax (Gibco) and 40 ng/mL human recombinant FGF (Peprotech) at in 5 % CO2, 37C°C incubator. .. Differentiation was initiated by adding to the media 1 μg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech), and 1 mM cAMP (Sigma).

Article Title: IGF2BPs directly regulate the noncanonical translation of toxic proteins from mutant FMR1 mRNA containing expanded CGG repeats
Article Snippet: For measuring the number of FMRpolyG inclusions experiment, control siRNA and siRNA targeting IGF2BP3 were delivered by lipofection using RNAiMAX (Thermo Fisher Scientific) every 7 days, starting from day 7 after beginning of differentiation. .. For immunofluorescence, iPSC-derived neurons were grown on poly-L-ornithine (Merck) and laminin (Merck) cell culture chamber slides (Ibidi). .. After 21 days of differentiation, cells were fixed in 4% paraformaldehyde/PBS for 15 min at room temperature, washed with PBS, and permeabilized in 0.5% Triton X-100/PBS and blocked by incubation for 45 min with 5% normal donkey serum (Jackson ImmunoResearch) 0.1% tween-20 in PBS.

Article Title: Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain
Article Snippet: Cell culture and siRNA delivery LUHMES cells were obtained from ATCC (CRL- 2927; RRID:CVCL_B056) and tested negative for mycoplasma contamination. .. Cells were cultured on 50 μg/mL Poly- L- ornithine (Merck) and 1 μg/mL human plasma fibronectin (Sigma) coated flasks and cultured in Advanced DMEM/F12 (Gibco) added with 1% N- 2 supplement (Gibco), GlutaMax (Gibco) and 40 ng/mL human recombinant FGF (Peprotech) at in 5 % CO2, 37C°C incubator. .. Differentiation was initiated by adding to the media 1 μg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech), and 1 mM cAMP (Sigma).

Clinical Proteomics:

Article Title: Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain
Article Snippet: LUHMES cells were obtained from ATCC (CRL-2927; RRID: CVCL_B056 ) and tested negative for mycoplasma contamination. .. Cells were cultured on 50 μg/mL Poly-L-ornithine (Merck) and 1 μg/mL human plasma fibronectin (Sigma) coated flasks and cultured in Advanced DMEM/F12 (Gibco) added with 1% N-2 supplement (Gibco), GlutaMax (Gibco) and 40 ng/mL human recombinant FGF (Peprotech) at in 5 % CO2, 37C°C incubator. .. Differentiation was initiated by adding to the media 1 μg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech), and 1 mM cAMP (Sigma).

Article Title: Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain
Article Snippet: Cell culture and siRNA delivery LUHMES cells were obtained from ATCC (CRL- 2927; RRID:CVCL_B056) and tested negative for mycoplasma contamination. .. Cells were cultured on 50 μg/mL Poly- L- ornithine (Merck) and 1 μg/mL human plasma fibronectin (Sigma) coated flasks and cultured in Advanced DMEM/F12 (Gibco) added with 1% N- 2 supplement (Gibco), GlutaMax (Gibco) and 40 ng/mL human recombinant FGF (Peprotech) at in 5 % CO2, 37C°C incubator. .. Differentiation was initiated by adding to the media 1 μg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech), and 1 mM cAMP (Sigma).

Recombinant:

Article Title: Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain
Article Snippet: LUHMES cells were obtained from ATCC (CRL-2927; RRID: CVCL_B056 ) and tested negative for mycoplasma contamination. .. Cells were cultured on 50 μg/mL Poly-L-ornithine (Merck) and 1 μg/mL human plasma fibronectin (Sigma) coated flasks and cultured in Advanced DMEM/F12 (Gibco) added with 1% N-2 supplement (Gibco), GlutaMax (Gibco) and 40 ng/mL human recombinant FGF (Peprotech) at in 5 % CO2, 37C°C incubator. .. Differentiation was initiated by adding to the media 1 μg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech), and 1 mM cAMP (Sigma).

Article Title: Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain
Article Snippet: Cell culture and siRNA delivery LUHMES cells were obtained from ATCC (CRL- 2927; RRID:CVCL_B056) and tested negative for mycoplasma contamination. .. Cells were cultured on 50 μg/mL Poly- L- ornithine (Merck) and 1 μg/mL human plasma fibronectin (Sigma) coated flasks and cultured in Advanced DMEM/F12 (Gibco) added with 1% N- 2 supplement (Gibco), GlutaMax (Gibco) and 40 ng/mL human recombinant FGF (Peprotech) at in 5 % CO2, 37C°C incubator. .. Differentiation was initiated by adding to the media 1 μg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech), and 1 mM cAMP (Sigma).

Immunofluorescence:

Article Title: IGF2BPs directly regulate the noncanonical translation of toxic proteins from mutant FMR1 mRNA containing expanded CGG repeats
Article Snippet: For measuring the number of FMRpolyG inclusions experiment, control siRNA and siRNA targeting IGF2BP3 were delivered by lipofection using RNAiMAX (Thermo Fisher Scientific) every 7 days, starting from day 7 after beginning of differentiation. .. For immunofluorescence, iPSC-derived neurons were grown on poly-L-ornithine (Merck) and laminin (Merck) cell culture chamber slides (Ibidi). .. After 21 days of differentiation, cells were fixed in 4% paraformaldehyde/PBS for 15 min at room temperature, washed with PBS, and permeabilized in 0.5% Triton X-100/PBS and blocked by incubation for 45 min with 5% normal donkey serum (Jackson ImmunoResearch) 0.1% tween-20 in PBS.



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